tag:blogger.com,1999:blog-3017697080484068141.post8662444974473612848..comments2023-10-08T04:37:34.635-07:00Comments on No DNA Control: Back to the grindChanghttp://www.blogger.com/profile/12291718994939895064noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-3017697080484068141.post-1044922377821220822009-08-12T14:07:28.222-07:002009-08-12T14:07:28.222-07:00Why not just use linearized pUSS1 for the cytosoli...Why not just use linearized pUSS1 for the cytosolic reisolation tests? How 'large' does it need to be?<br /><br />I would use the simplest already-available DNA fragment for these tests. If you need a 86-028NP chromosomal fragment for other tests, optimize it for those tests.<br /><br />Can we (you) think up a clever test molecule for the cytosolic reisolation tests? It probably should share no sequences with the KW20 chromosome, so you can be sure that what you've isolated is your input DNA.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.com